杜文静，女，博士生导师

2003-2008年，中国科学技术大学生命科学学院，博士学位

2008-2013年，美国宾夕法尼亚大学医学院，博士后

2013-2015年，美国宾夕法尼亚大学医学院，研究助理

2016年1月起，中国医学科学院基础医学研究所，课题组长，博士生导师

2016年入选国家海外引进高层次人才青年项目

2019年7月起聘为北京协和医学院准聘副教授

课题组研究方向：细胞代谢调控与代谢物感知。（1）研究不同生理及病理条件下细胞代谢途径的调控机制；（2）关键代谢物的感知机制与新功能探索。主要利用细胞、生化、分子生物学手段和小鼠模型来探索不同生理及病理条件下代谢调控的规律，揭示多种重大代谢疾病中如糖尿病、脂肪肝、癌症、心血管疾病等代谢紊乱的原因及其致病机制，通过研究希望能为重大代谢疾病的治疗发现新策略或新靶点。

主要研究成果：在Cell Reports (2023), EMBO Journal (2023), PNAS (2023), Nature Metabolism (2022和 2021), Nature Communications (2018), EMBO Rep (2022和 2021)，Science Bulletin (2019)，JBC (2017), Nature (2013), Nature Cell Biology (2013, 2011)等期刊发表学术论文20余篇。

课题组成员：

（1）      工作人员：李莉 助理研究员；李薇 主管技师

（2）      直博生：张振西（毕业于华中农业大学）司玙璠（毕业于中国农业大学）

       陈 杨（毕业于北京大学医学部）

（3）      博士生：郭 唱（毕业于四川大学）

（4）      硕士生：李皓月（毕业于南京中医药大学）程润宁（毕业于苏州大学）

       袁嘉阳（毕业于哈尔滨医科大学）

（5）      协和4+4临床试点班 ：秋昱翀（毕业于北京大学）

已毕业学生：

杨延婷（2023年博士毕业）

苏靓誉（2023年硕士毕业）柳子朝（2023年硕士毕业）

寇俊婕（2022年博士毕业）康有力（2022年硕士毕业）

杨 帆（2021年硕士毕业）

孙会姗（2020年博士毕业）赵 丹（2020年硕士毕业）

课题组招聘：

课题组长期招聘硕士/博士研究生，欢迎对本课题组研究方向感兴趣的同学报考。

课题组招聘1-2名助理/副研究员，1-2名博士后。需熟练掌握分子生物学与生物化学实验技术，细胞培养，小鼠模型建立等，能够在课题组长的指导下开展课题研究与基金申请工作，协助课题组长指导研究生。

联系方式：

中国医学科学院基础医学研究所，细胞生物学系，可胜大楼457、258

北京市东城区东单三条5号，邮编：100005

电话：010-69156953

Email: wenjingdu@ibms.pumc.edu.cn;  wjdu123@hotmail.com

课题组照片：

代表论文：

1)      Yang Yanting, Zhang Zhenxi, Li Wei, Si Yufan, Li Li, Du Wenjing*. αKG-driven RNA polymerase II transcription of cyclin D1 licenses malic enzyme 2 to promote cell-cycle progression. Cell Reports, 2023, Jul 25;42(7):112770.

2)      Li Wei#, Kou Junjie#, Zhang Zhenxi, Li Haoyue, Li Li, Du Wenjing*. Cellular redox homeostasis maintained by malic enzyme 2 is essential for MYC-driven T-cell lymphomagenesis. PNAS, 2023, Jun 6;120(23):e2217869120.

3)      Yang Yanting, Zhang Zhenxi, Li Wei, Li Li, Zhou Ying, Du Wenjing*. ME2 Promotes Hepatocellular Carcinoma Cell Migration through Pyruvate. Metabolites. 2023 Apr 10;13(4):540.

4)      Yao Pengbo, Zhang Zhenxi, Liu Hongchao, Jiang Peng, Li Wei, Du Wenjing*. p53 protects against alcoholic fatty liver disease via ALDH2 inhibition. EMBO Journal, 2023 Apr 17;42(8):e112304.

5)      Yao Pengbo#, Chen Taiqi#, Jiang Peng, Li Li, Du Wenjing*. Functional skewing of TRIM21-SIRT5 interplay dictates IL-1β production in DSS-induced colitis. EMBO Reports. 2022 Jun 30;e54391. doi: 10.15252/embr.202154391.

6)      Zhang Zhenxi, Du Wenjing*. Malic enzyme 2 as a therapeutic target for cancer: comments on 'Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate'. J Mol Cell Biol. 2022 Apr 18;mjac024. doi: 10.1093/jmcb/mjac024.

7)      Zhao Mengjia#, Yao Pengbo#, Mao Youxiang#, Wu Jinjun#, Wang Weihua, Geng Chenhui, Cheng Jie, Du Wenjing*, Jiang Peng*. Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate. Nature Metabolism. 2022 Feb;4(2):225-238

8)      Sun Huishan#, Li Li#, Li Wei#, Yang Fan, Zhang Zhenxi, Liu Zizhao, Du Wenjing*. p53 transcriptionally regulates SQLE to repress cholesterol synthesis and tumor growth. EMBO Reports. 2021 Aug 30:e52537. doi: 10.15252/embr.202152537

9)      Li Wei #; Kou Junjie #; Qin Junyin #; Li Li; Zhang Zhenxi; Pan Ying; Xue Yi *; Du Wenjing*; NADPH levels affect cellular epigenetic state by inhibiting HDAC3–Ncor complex. Nature Metabolism, 2021, 3(1): 75-89.

10)      Yang Fan#; Kou Junjie#; Li Wei; Du Wenjing*; MYC Enhances Cholesterol Biosynthesis and Supports Cell Proliferation Through SQLE, Frontiers in Cell and Developmental Biology, 2021, 9:655889

11)      Zhang Zhenxi, Su Jingyu, Li Li*, Du Wenjing*. Identification of precise therapeutic targets and characteristic prognostic genes based on immune gene characteristics in uveal melanoma. Frontiers in Cell and Developmental Biology, 2021, doi: 10.3389/fcell.2021.666462

12)      Zhao Mengjia; Jiang Peng; Du Wenjing*; MYC retards cancer cell migration through suppressing fibronectin expression, Science Bulletin, 2019, 64(11): 715-717. 

13)      Li, Le#; Li, Lijia#; Li, Wei; Chen, Taiqi; Bin Zou; Zhao, Lina; Wang, Huili; Wang, Xueying; Xu, Lina; Liu, Xiaohui; Wang, Dong; Li, Bo; Mak, Tak W; Du, Wenjing*; Yang, Xiaolu*; Jiang, Peng*; TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell proliferation., Nature Communications, 2018, 9(1): 4683-4683. 

14)      Yao, Pengbo; Sun, Huishan; Xu, Chang; Chen, Taiqi; Zou, Bing; Jiang, Peng*; Du, Wenjing*; Evidence for a direct cross-talk between malic enzyme and the pentose phosphate pathway via structural interactions, Journal of Biological Chemistry, 2017, 292(41): 17113-17120. 

15)      Du, Wenjing#; Jiang, Peng#; Mancuso, Anthony; Stonestrom, Aaron; Brewer, Michael D; Minn, Andy J; Mak, Tak W; Wu, Mian*; Yang, Xiaolu*; TAp73 enhances the pentose phosphate pathway and supports cell proliferation., Nat Cell Biol, 2013, 15(8): 991-1000.  Notes: cover story and featured in News & Views in this issue.

16)      Jiang, Peng#; Du, Wenjing#; Mancuso, Anthony; Wellen, Kathryn E; Yang, Xiaolu*; Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence., Nature, 2013, 493(7434): 689-693.  Notes: Highlighted in journal Cell Research.

17)      Jiang, Peng#; Du, Wenjing#; Wang, Xingwu; Mancuso, Anthony; Gao, Xiang; Wu, Mian*; Yang, Xiaolu*; p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase., Nat Cell Biol, 2011, 13(3): 310-316. Notes: cover story and featured in News & Views in this issue. Highlighted in Nature Chemical Biology.

18)      Du, Wenjing#; Jiang, Peng#; Li, Nan; Mei, Yide; Wang, Xingwu; Wen, Longping; Yang, Xiaolu*; Wu, Mian*; Suppression of p53 activity by Siva1., Cell Death Differ, 2009, 16(11): 1493-1504.

19)      Jiang, Peng#; Du, Wenjing#; Heese, Klaus; Wu, Mian*; The Bad guy cooperates with good cop p53: Bad is transcriptionally up-regulated by p53 and forms a Bad/p53 complex at the mitochondria to induce apoptosis., Mol Cell Biol, 2006, 26(23): 9071-9082. 

20)      Li Le; Mao Youxiang; Zhao Lina; Li Lijia; Wu Jinjun; Zhao Mengjia; Du Wenjing; Yu Li; Jiang Peng*; p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis, Nature, 2019, 567(7747): 253-+.  

21)      Jiang, Peng*; Du, Wenjing; Wu, Mian*; Regulation of the pentose phosphate pathway in cancer., Protein Cell, 2014, 5(8): 592-602. 

22)      Jiang, Peng; Du, Wenjing; Yang, Xiaolu*; A critical role of glucose-6-phosphate dehydrogenase in TAp73-mediated cell proliferation., Cell Cycle, 2013, 12(24): 3720-3726. 

23)      Jiang, Peng; Du, Wenjing; Yang, Xiaolu*; p53 and regulation of tumor metabolism., J Carcinog, 2013, 12: 21-21. 

24)      Wang, Xingwu; Zha, Meng; Zhao, Xiaocheng; Jiang, Peng; Du, Wenjing; Tam, Andrew Y H; Mei, Yide*; Wu, Mian*; Siva1 inhibits p53 function by acting as an ARF E3 ubiquitin ligase., Nat Commun, 2013, 4: 1551-1551.

25)      Li, Nan; Jiang, Peng; Du, Wenjing; Wu, Zhengsheng; Li, Cong; Qiao, Mengran; Yang, Xiaolu*; Wu, Mian*; Siva1 suppresses epithelial-mesenchymal transition and metastasis of tumor cells by inhibiting stathmin and stabilizing microtubules., Proc Natl Acad Sci U S A, 2011, 108(31): 12851-12856. 

26)      Wu, Jiawen; Yang, Yinshan; Zhang, Jiahai; Ji, Peng; Du, Wenjing; Jiang, Peng; Xie, Dinghai; Huang, Hongda; Wu, Mian; Zhang, Guangzhao; Wu, Jihui*; Shi, Yunyu*; Domain-swapped dimerization of the second PDZ domain of ZO2 may provide a structural basis for the polymerization of claudins., J Biol Chem, 2007, 282(49): 35988-35999. 

27)      Jiang, Peng; Du, Wenjing; Wu, Mian*; p53 and Bad: remote strangers become close friends., Cell Res, 2007, 17(4): 283-285. 

28)      Ma, L; Huang, Y; Song, Z; Feng, S; Tian, X; Du, W; Qiu, X; Heese, K; Wu, M*; Livin promotes Smac/DIABLO degradation by ubiquitin-proteasome pathway., Cell Death Differ, 2006, 13(12): 2079-2088. 

29)      Mei, Yide; Du, Wenjing; Yang, Yonghui; Wu, Mian*; Puma(*)Mcl-1 interaction is not sufficient to prevent rapid degradation of Mcl-1., Oncogene, 2005, 24(48): 7224-7237.